• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Measurement of in vivo rectal mucosal cytokine and eicosanoid production in ulcerative colitis using filter paper
【2h】

Measurement of in vivo rectal mucosal cytokine and eicosanoid production in ulcerative colitis using filter paper

机译：使用滤纸测量溃疡性结肠炎体内直肠粘膜细胞因子和类花生酸的产生

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND—Excessive mucosal generation of cytokines and eicosanoids has been reported in vitro in ulcerative colitis (UC) using traumatising biopsy techniques, and in vivo using time consuming rectal dialysis.
AIMS—To validate a simple filter paper technique to profile rectal mucosal production of cytokines and eicosanoids in vivo in patients with UC compared with controls.
PATIENTS—Forty one patients with UC (21 with active disease) and 16 controls were studied.
METHODS—In vitro, recovery of known concentrations of cytokine or mediator applied to filter papers was measured by ELISA following incubation in buffer. In vivo, patients and controls had filter papers apposed to the rectal mucosa briefly through a rigid sigmoidoscope. Filter papers were then incubated prior to assay by ELISA.
RESULTS—In vitro validation studies showed that the filter paper technique could be used to measure mucosal release of interleukin-1β (IL-1β), tumour necrosis factor α (TNF-α), thromboxane B2 (TXB2), and prostaglandin E2 (PGE2), but not interferon γ (IFN-γ). Mucosal release of IL-1β, TNF-α, TXB2 and PGE2 were significantly increased in active UC (p=0.001) and correlated directly with disease activity (p=0.02).
CONCLUSIONS—The filter paper technique confirmed increased rectal mucosal release of cytokines and eicosanoids in UC, in proportion to disease activity. The simplicity, safety and speed of the technique make it a practicable option for use in the outpatient clinic to study the pathogenesis of inflammatory bowel disease, and potentially its response to treatment.


Keywords: cytokines; eicosanoids; ulcerative colitis; rectal dialysis
机译：背景技术已有报道，在体外使用溃疡性活检技术在溃疡性结肠炎（UC）中以及在体内使用耗时的直肠透析在细胞膜和类二十烷酸中的粘膜生成过多。目的—为了验证一种简单的滤纸技术，与对照组相比，UC患者体内直肠黏膜细胞因子和类二十烷酸生成的概况。患者-研究了41例UC患者（21例患有活动性疾病）和16例对照。方法—在体外，在缓冲液中孵育后，通过ELISA测定应用于滤纸的已知浓度的细胞因子或介质的回收率。在体内，患者和对照者通过刚性乙状结肠镜短暂地将滤纸贴在直肠粘膜上。然后将滤纸温育，然后通过ELISA测定。结果—体外验证研究表明，滤纸技术可用于测量白介素-1β（IL-1β），肿瘤坏死因子α（TNF-α），血栓素B2（TXB2）和前列腺素E2（PGE2）的粘膜释放），但不干扰素γ（IFN-γ）。 IL-1β，TNF-α，TXB2和PGE2的粘膜释放在活动性UC中显着增加（p = 0.001），并与疾病活动直接相关（p = 0.02）。结论：滤纸技术证实了直肠粘膜释放的细胞因子和类花生酸类药物与疾病活动成正比。该技术的简单性，安全性和速度使其成为在门诊研究炎症性肠病的发病机理以及对治疗的潜在反应的可行选择。关键词：细胞因子；类花生酸;溃疡性结肠炎;直肠透析

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号